Production of capsular polysaccharide of Streptococcus pneumoniae type 14 and its purification by affinity chromatography.
نویسندگان
چکیده
We describe a rapid and efficient method for producing the capsular polysaccharide of Streptococcus pneumoniae by fermentation on tryptic soy broth and purification of this compound by using immobilized soybean lectin as an affinity adsorbent. In principle, the same strategy can be used to produce purified capsular polysaccharides from other streptococcal serotypes by selecting the appropriate lectin adsorbents.
منابع مشابه
Prokaryotic expression, purification and immunogenicity analysis of CpsD protein from Streptococcus iniae
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متن کاملProkaryotic expression, purification and immunogenicity analysis of CpsD protein from Streptococcus iniae
Streptococcus iniae is a major cause of serious bacterial infections in both fish and human beings. Capsular polysaccharide (CPS) of S. iniae is vital to evade phagocytic clearance of the host and serves as an important protective antigen of S. iniae infection in aquatic animals. The CpsD gene was determined to be highly conservative in capsule polysaccharide operon. Prokaryotic expression of t...
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 67 2 شماره
صفحات -
تاریخ انتشار 2001